Separate highly hydrophilic molecules by hydrophilic interaction liquid chromatography using Thermo Scientific™ Acclaim HILIC-10 columns.
These columns retain and separate highly polar molecules that are unretained by reversed-phase chromatography. Their high-purity, spherical, porous silica base is covalently modified with a proprietary hydrophilic layer. This chemistry enables the use of 5 to 20% aqueous mobile phase while maintaining affinity for polar analytes. Sample throughput is increased without the need to evaporate and reconstitute samples dissolved in nonaqueous solvents.
Ordering information: Guard Cartridges require use of the corresponding guard holder. See Acclaim Guard Kit Holder and Coupler V-2 (VWR Part No. 10046-750).