Human mobilized peripheral blood CD133 stem/progenitor cells are isolated from peripheral blood mononuclear cells (PBMCs) using positive immunomagnetic cell separation procedures.
PBMCs are collected from healthy human volunteer donors under IRB approved informed consent by leukapheresis using the COBE® Spectra Apheresis System MNC collection protocol. Donors are injected with G-CSF (Neupogen® - FDA approved), which stimulates the bone marrow to produce a large number of hematopoietic and progenitor stem cells and mobilizes them into the peripheral blood stream. This mononuclear cell-rich leukapheresis product (LeukoPak) is extracted using continuous flow centrifugal technology directly into the collection bag. Mononuclear cells are purified using density gradient centrifugation techniques.
Serological and Infectious disease testing are performed with each donation. Customers should handle product as if potentially infectious following biosafety level 2 procedures.