Alcian blue solution used in nuclear staining, for histology, Sigma-Aldrich®

Supplier: MilliporeSigma

Synonyms: C.I. 74240C.N. AnAn-6Ingrain Blue 1Alcian blue 8 GX

1.01647.0500 1.01647.0500
EM1.01647.0500CS 4300.39 USD
CA1.01647.0500 EM1.01647.0500
Alcian blue solution used in nuclear staining, for histology, Sigma-Aldrich®
Alcian blue 8 GX

Used to stain acidic mucosubstances

The combination of PAS staining with Alcian blue allows (at pH 2.5) the additional identification of acidic mucosubstances (glycosaminoglycans). PAS staining is used for paraffin sections and cell smears, and upon use of the ready-to-use Alcian blue staining solution, nuclei will appear blue, acidic mucosubstances light blue and polysaccharides as well as neutral mucopolysaccharides will be stained purple. Additionally, the Alcian blue staining solution (at pH 2.5) allows the identification of acidic mucosubstances without differentiation of carboxyl and sulfate groups, upon which the nuclei are counter-stained with nuclear fast red. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations.

Formula: C₅₆H₆₈Cl₄CuN₁₆S₄
MW: 1298.86 g/mol
Density: 1.006 g/cm3 (20 °C)
Storage Temperature: Ambient
MDL Number: MFCD00010720
CAS Number: 33864-99-2

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Specification Test Results

Suitability for microscopy passes test
neutral mucopolysaccharides purple
polysaccharides purple
PAS negative acidic mucosubstances light blue
Nuclei blue

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