Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific

Supplier: Thermo Scientific
Pierce
90082 90078 90080 90082 78243 78248 78266 90084 90079 78243 90078 78266 90083 78248 90080 90083 78260 90079 90084 78260
PI90082EA 72.78 USD InStock
PI90082 PI90078 PI90080 CAPI90082 CAPI78243 CAPI78248 CAPI78266 CAPI90084 PI90079 PI78243 CAPI90078 PI78266 PI90083 PI78248 CAPI90080 CAPI90083 CAPI78260 CAPI90079 PI90084 PI78260
Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific
Protein Reagents Protein Extraction Reagents
Thermo Scientific B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells without harsh chemicals or mechanical disruption.

  • Lysis of bacteria occurs directly in cell culture media
  • No centrifugation steps
  • Compatible with the addition of protease inhibitors, and the resulting protein extract can be used in protein assays, typical affinity purification methods (eg. GST, 6XHis), and other applications

These easy-to-use cell lysis reagents are nonionic detergent solutions that effectively disrupt cells and solubilize native or recombinant proteins without denaturation.

Certain formulations include DNase I and lysozyme enzyme supplements to improve yield of large molecular weight proteins that are difficult to purify. B-PER Reagents are compatible with the addition of standard protease inhibitors and with downstream protein purification methods, such as GST purification and His-tagged immobilized metal affinity chromatography (IMAC).

Delivery information: Kit contains B-PER® Reagent, 250 mL; Lysozyme, 50 mg/mL, 0.5 mL; and DNase I, 2500 U/mL, 0.5 mL. Separate reagents are also available.
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