Pierce™ Bacterial Protein Extraction Reagents, Thermo ScientificSupplier: Thermo Scientific
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PI90082EA 72.78 USD InStockPI90082 PI90078 PI90080 CAPI90082 CAPI78243 CAPI78248 CAPI78266 CAPI90084 PI90079 PI78243 CAPI90078 PI78266 PI90083 PI78248 CAPI90080 CAPI90083 CAPI78260 CAPI90079 PI90084 PI78260
Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific
Protein Reagents Protein Extraction Reagents
Thermo Scientific B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells without harsh chemicals or mechanical disruption.
- Lysis of bacteria occurs directly in cell culture media
- No centrifugation steps
- Compatible with the addition of protease inhibitors, and the resulting protein extract can be used in protein assays, typical affinity purification methods (eg. GST, 6XHis), and other applications
These easy-to-use cell lysis reagents are nonionic detergent solutions that effectively disrupt cells and solubilize native or recombinant proteins without denaturation.
Certain formulations include DNase I and lysozyme enzyme supplements to improve yield of large molecular weight proteins that are difficult to purify. B-PER Reagents are compatible with the addition of standard protease inhibitors and with downstream protein purification methods, such as GST purification and His-tagged immobilized metal affinity chromatography (IMAC).
Delivery information: Kit contains B-PER® Reagent, 250 mL; Lysozyme, 50 mg/mL, 0.5 mL; and DNase I, 2500 U/mL, 0.5 mL. Separate reagents are also available.