Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific

Supplier: Thermo Fisher Scientific
90082 90078 90082 78243 78248 78266 90079 78243 90078 78266 90083 78248 78260 90079 90084 78260
PI90084EA 317.91 USD
CAPI78266 PI90084 CAPI78243 PI90082 CAPI78248 PI90083 CAPI78260 CAPI90082 CAPI90078 PI78243 CAPI90079 PI78260 PI78248 PI90079 PI78266 PI90078
Pierce™ Bacterial Protein Extraction Reagents, Thermo Scientific
Protein Extraction Protein Extraction Reagents
Thermo Scientific B-PER Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells without harsh chemicals or mechanical disruption.

  • Lysis of bacteria occurs directly in cell culture media
  • No centrifugation steps
  • Compatible with the addition of protease inhibitors, and the resulting protein extract can be used in protein assays, typical affinity purification methods (eg. GST, 6XHis), and other applications

These easy-to-use cell lysis reagents are nonionic detergent solutions that effectively disrupt cells and solubilize native or recombinant proteins without denaturation.

Certain formulations include DNase I and lysozyme enzyme supplements to improve yield of large molecular weight proteins that are difficult to purify. B-PER Reagents are compatible with the addition of standard protease inhibitors and with downstream protein purification methods, such as GST purification and His-tagged immobilized metal affinity chromatography (IMAC).

Delivery information: Kit contains B-PER® Reagent, 250 mL; Lysozyme, 50 mg/mL, 0.5 mL; and DNase I, 2500 U/mL, 0.5 mL. Separate reagents are also available.
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