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Percoll PLUS® Density Gradient Media, GE Healthcare

Supplier: GE Healthcare - Life Sciences
Percoll® PLUS is for preparation of cells, subcellular particles and larger viruses.

  • Iso-osmotic gradients throughout cover a range of densities up to 13 g/ml
  • Available in easy-to-open, resealable 250 ml and 1 l bottles
  • Percoll® PLUS has verified low endotoxin level
  • Non-toxic and re-sterilizable

Gradients can either be preformed or spontaneously generated by centrifugation at moderate speeds in an angle-head rotor. Iso-osmotic gradients throughout cover a range of densities up to 1.3 g/ml. Low viscosity allows cell isolations on preformed gradients in only a few minutes using low centrifugal forces (200 to 1000 x g).

Percoll® PLUS is non-toxic, almost chemically inert, and does not adhere to membranes. Percoll® PLUS can be stored unopened for at least five years. Preformed gradients can be stored for weeks without change in gradient shape, provided that the gradient remains unfrozen. Percoll® PLUS can be buffered within the pH range 5.5 to 10 without change in properties. Both concentrated and diluted Percoll PLUS can be resterilized by autoclaving for 30 min at 120°C. Used in density centrifugation applications for the isolation and purification of cells, subcellular particles and viruses down to ~70S. Used to produce preformed, continuous or discontinuous gradients. Liquid/suspension physical state. Physical form is a colloidal solution of silica particles coated with silane. Colorless to slightly yellow. Certificate of Analysis is available.



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Description Size Supplier No. VWR Catalog Number Unit Price Quantity
Percoll® PLUS 250 mL 17-5445-02 89428-528 Each (250ml) Retrieving
Percoll® PLUS 1 L 17-5445-01 89428-530 Each (1l) Retrieving
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SPECIFICATIONS

Density 1.125 g/ml; 1.135 g/ml (Max.)
Stability Provided as solution which is stable for at least 5 yr when stored between 4°C and 30°C
pH Range 8.9–9.9
Viscosity 15 cP (Max.)
Shelf Life 5 years (Estimated Shelf Life from Manufacture Date)
Carbon Content 0.04-5.5% (in Dry Residue)
Osmolality < 30 mOsm/kg \< 30 mOsm/kg
Radius ~15 nm Stokes Radius Min.; ~30 nm Stokes Radius Max.
Storage Conditions 4–30°C
Endotoxin Activity 2 EU/ml (Max.)
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