SNAP-CELL® Fluorescein , New England Biolabs

S9107S S9107S
101447-664EA 314.68 USD
CA101447-664 101447-664
SNAP-CELL® Fluorescein , New England Biolabs
SNAP-Cell® Fluorescein is a green fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells or in vitro.

This cell-permeable substrate (BG-Fluorescein) is based on diacetylfluorescein and is suitable for standard fluorescein filter sets. Diacetylfuorescein is essentially non-fluorescent, but it becomes fluorescent inside the cell when it is hydrolyzed by non-specific esterases, yielding fluorescein. It has an excitation maximum at 500 nm and an emission maximum at 532 nm. This substrate has limited photostability. If this presents a problem, we recommend using SNAP-Cell 505, which has similar spectral characteristics but much greater photostability. This package contains 50 nmol of SNAP-Cell Fluorescein substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.
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