Phusion High-Fidelity DNA Polymerase, New England BiolabsSupplier: New England Biolabs (NEB)
M0530S M0530L M0530S M0530L
101641-014EA 113.78 USD InStockCA101641-014 CA101641-012 101641-014 101641-012
Phusion High-Fidelity DNA Polymerase, New England Biolabs
Nucleic Acid Reagents PCR Reagents Polymerases
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification.
Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products. Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates.