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ONETAQ® RT-PCR Kit, New England Biolabs

Supplier: New England Biolabs

OneTaq RT-PCR Kit - 30 rxns

OneTaq RT-PCR Kit - 30 rxns

ONETAQ®

E5310S

101710-294EA 159.4 USD

101710-294

ONETAQ® RT-PCR Kit, New England Biolabs

Nucleic Acid Reagents End-point PCR Enzymes and Kits

OneTaq® RT-PCR Kit combines two powerful mixes, M-MuLV Enzyme Mix and OneTaq Hot Start 2X Master Mix with Standard Buffer for 2-step RT-PCR applications.

The two mixes require minimal handling during reaction setup and yet offer consistent and robust RT-PCR reactions. The first strand cDNA synthesis is achieved by using two optimized mixes, M-MuLV Enzyme Mix and M-MuLV Reaction Mix. M-MuLV Enzyme Mix combines M-MuLV Reverse Transcriptase and murine RNase Inhibitor while M-MuLV Reaction Mix contains dNTPs and an optimized buffer. The kit also contains two optimized primers for reverse transcription and nuclease-free water. An anchored oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail. The optimized Random Primer Mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs.

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