M13K07 Helper Phage, New England Biolabs

Supplier: New England Biolabs
N0315S N0315S
101228-402EA 128 USD
CA101228-402 101228-402
M13K07 Helper Phage, New England Biolabs
Vectors, Plasmids and Libraries
M13KO7 is an M13 derivative which carries the mutation Met40Ile in gII, with the origin of replication from P15A and the kanamycin resistance gene from Tn903 both inserted within the M13 origin of replication

M13KO7 is able to replicate in the absence of phagemid DNA. In the presence of a phagemid bearing a wild-type M13 or f1 origin, single-stranded phagemid is packaged preferentially and secreted into the culture medium. This allows easy production of single-stranded phagemid DNA for mutagenesis or sequencing.
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