Instant Sticky-end Ligase Master Mix, New England Biolabs

Supplier: New England Biolabs
M0370L M0370S M0370L M0370S
102715-946EA 108 USD
CA102715-944 102715-946 102715-944 CA102715-946
Instant Sticky-end Ligase Master Mix, New England Biolabs
Nucleic Acid Reagents Cloning Kits and Ligation
Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA ligase and a proprietary ligation enhancer in an optimized reaction buffer.

Specifically formulated to rapidly ligate cohesive-end (2–4 bp) substrates and improve transformation, the mix simplifies reaction set-up and provides an optimized ratio of enzyme and buffer components. No thawing of the master mix is required as it maintains a liquid state during storage at -20°C and no incubation time is necessary to achieve ligation efficiencies sufficient for successful sub-cloning of sticky-end substrates. Just add the master mix to DNA with compatible ends, mix and transform; thereby reducing the valuable time needed for routine ligations. Ligations for subcloning can be carried out in small volumes with low concentrations, allowing users to conserve precious DNA samples, and be used directly to transform many strains of chemically competent E. coli without dilution.
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