GSTrap HP Columns, GE HealthcareSupplier: GE Healthcare - Life Sciences
17-5281-01 17-5282-01 17-5282-02 17-5282-02 17-5282-01 17-5281-01
95056-020PK 1534.01 USD InStockCA95056-212L CA95056-018L 95056-020 CA95056-020L 95056-018 95056-212
GSTrap HP Columns, GE Healthcare
GSTrap HP columns are prepacked with Glutathione Sepharose High Performance medium and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins
- High resolution purification of GST-tagged proteins
- Mild elution conditions preserving protein antigenicity and function
- Fast, one-step purification
- Easy to use with syringe, pump or a chromatography system
GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.
GSTrap HP 1-ml and 5-ml columns are prepacked with Glutathione Sepharose High Performance medium, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose High Performance. GST-tagged proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer.