Fluorescein-5-maleimide ≥90% (by HPLC), Ultrapure
Supplier: Enzo Life Sciences
Synonyms:
1-(3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)-1H-pyrrole-2,5-dione
, N-(5-Fluoresceinyl)-maleimide
, 1-(3',6'-Dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthen]-5-yl)-1H-pyrrole-2,5-dione
, 5-Maleimidofluorescein
Thiol dye.
Fluorescein-5-maleimide enables protein labeling via thiol modifications in most proteins at cysteine residues that either are intrinsically present or resulted from reduction of cystine residues. Unlike iodoacetamides, maleimides do not react with histidine and methionine under physiological conditions. The probe is suitable for assessing conformation- dependent accessibility of cysteine residues in proteins. Wavelength Maxima: Excitation 493 nm, Emission 515 nm.
Replaces former ENZ-52505
Formula:
C₂₄H₁₃NO₇ MW: 427.37 g/mol Storage Temperature: Freezer |
MDL Number:
MFCD00077345 CAS Number: 75350-46-8 |
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