The BioArray™ 3'-OH Terminal Labeling System is the gold standard end-labeling system for use with Affymetrix® DNA SNP, Resequencing and Prokaryotic microarrays.
The BioArray™ Terminal Labeling Kit with Biotin-ddUTP has been developed for DNA probe array assays. This method uses Bio-ddUTP and terminal deoxynucleotide transferase to catalyze the addition of a single biotin-ddUMP to the 3'-OH terminus of an amplified and fragmented target DNA molecule. A standard reaction will label up to 100 picomoles (equivalent to 1µg of a 30-nucleotide sequence). When DNA fragments are labeled at the 3'-OH terminus, the sequence bias that occurs with either nick translation or random priming is eliminated.
Ordering information: Quantity: 25 reactions Contents:
Biotin-ddUTP (100X), 25 μl
5X Reaction Buffer, 500 μlPotassium cacodylate buffer, pH 7, containing beta-mercaptoethanol and stabilizer
10X CoCl2 Solution, 250 μl
Terminal Deoxynucleotide Transferase (50X), 50 μl in storage buffer
