PATHO-GENE® AP-NBT/BCIP HPV in situ Typing Assay (Types 6/11, 16/18 and 31/33/51), Enzo Life Sciences

Supplier: Enzo Life Sciences
ENZ-32895
89165-710EA 1864.01 USD
89165-710
PATHO-GENE® AP-NBT/BCIP HPV in situ Typing Assay (Types 6/11, 16/18 and 31/33/51), Enzo Life Sciences
Assays Nucleic Acid Assays
The PathoGene® Human Papillomavirus (HPV) in situ typing assay for tissue sections provides reagents and materials for (a) preparation and pretreatment and (b) hybridization/detection and typing of HPV DNA (using alkaline phosphatase-NBT/BCIP for signal generation) in 10 tissue biopsy specimens.

The PathoGene® Human Papillomavirus (HPV) in situ typing assay for tissue sections employs separate mixtures of HPV-specific probes to identify HPV types 6/11, 16/18, or 31/33/51. HPV types 6 and 11 are primarily associated with benign lesions presenting as condylomas and mild dysplasia. HPV types 16 and 18 are more commonly associated with cervical intraepithelial neoplasia (CIN) and carcinoma in situ (CIS).

HPV types 31, 33 or 51 can be associated with either condyloma or CIN and CIS, presumably dependent upon other, as yet undefined, contributing factors or agents.

The PathoGene® Human Papillomavirus (HPV) in situ typing assay for tissue sections can be used to detect and identify HPV DNA in tissue sections under conditions that preserve cellular morphology.

Delivery information: Contents:
HPV 6/11 Probe Mix, 0.5 ml (pink) Mixture of biotin-labeled HPV 6 and HPV 11-specific probes in buffered formamide and hybridization enhancers
HPV 16/18 Probe Mix, 0.5 ml (purple) Mixture of biotin-labeled HPV 16 and HPV 18-specific probes in buffered formamide and hybridization enhancers
HPV 31/33/51 Probe Mix, 0.5 ml (green) Mixture of biotin-labeled HPV 31, HPV 33 and HPV 51-specific probes in buffered formamide and hybridization enhancers
Hybridization Wash Reagent, 15 ml (white) Buffered formamide
AP Detection Reagent, 15 ml (orange) Streptavidin-biotinylated alkaline phosphatase in buffered saline with stabilizers and preservative
NBT Reagent, 0.4 ml (amber) Nitroblue tetrazolium in solvent
BCIP Reagent, 0.4 ml (amber) Bromochloroindolyl phosphate in solvent
AP Reaction Buffer, 2×10 ml (gray) Buffered saline and alkaline phosphatase enhancers
Counterstain Concentrate, 30 ml Eosin Y (certified) in alcohol
Proteinase K, 5 mg Lyophilized powder
SignaSure® Wash Buffer Salts, 4 packets Each packet yields 1 liter of buffer
Pretreated Specimen Slides, 20 slides10 single-well plus 10 double-well slides for mounting formalin-fixed paraffin-embedded tissue specimens

Packaging: HPV 16 Probe Control Slide, Double-well slide with fixed HPV 16-positive cells.

Caution: This product is for research use only and is not to be used for diagnostic procedures.
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