UbiQapture-Q® Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences
UW8995-0001
89165-298EA 620.85 USD
89165-298
UbiQapture-Q® Kit, Enzo Life Sciences
Market-leading tool for isolation and detection of ubiquitinylated proteins.

1. Isolation and detection of the full range of ubiquitinylated protein conjugates (mono-/multi-/poly-ubiquitin modified, lysine linkage independent) from specific cell/tissue lysates of interest, especially low abundance (regulatory) proteins involved in the ubiquitin-proteasome pathway. 2. Capture and analysis of specific ubiquitinylated protein conjugates of interest from particular cell/tissue lysates. 3. Purification/pull down of ubiquitinylated proteins from cell free in vitro assays; for example to isolate Ub-p53 species from ubiquitinylation assays mediated by HeLa S100 lysate (Prod. No. BML-SW8750). 4. Release of free proteins in their active/native form by cleavage of ubiquitin/ubiquitin chains from the UbiQapture-Q matrix using deubiquitinylating enzymes such as USP2 (Prod. No. BML-UW9850). 5. Release of ubiquitinylated proteins in their active/native form by elution from the UbiQapture-Q matrix using, for example, high salt buffer. Note: Protocol provided covers applications 1-3. Assay set-up can be readily modified for alternative applications by inclusion, omission or substitution of specific components. Kit is not suitable for use with purified proteins

For isolation and enrichment of ubiquitinylated proteins.The UbiQapture™-Q Kit is an efficient tool for the selective isolation of ubiquitinylated proteins. The Kit facilitates the isolation of both mono- and poly-ubiquitinylated proteins (independent of lysine residue chain linkage) from cell extracts, tissue lysates and in vitro assay solutions through use of a high-binding affinity matrix. Captured proteins can be analyzed by Western blotting using the highly sensitive ubiquitin-conjugate specific antibody provided, using antibodies to specific proteins of interest, or eluted from the matrix for subsequent biochemical characterization. The UbiQapture™-Q matrix supplied with the Kit has superior binding characteristics compared to other commercially available matrices and is compatible with a wide range of lysate buffers and cell/tissue samples from a variety of species.Suggested uses: Isolation and detection of ubiquitinylated protein conjugates from a specific cell/tissue lysate, Capture and analysis of specific ubiquitinylated protein conjugates of interest from particular cell/tissue lysates, Release of free proteins in their active/native form by cleavage of ubiquitin/ubiquitin chains from the UbiQapture™-Q matrix using a deubiquitinylating enzyme, Release of ubiquitinylated proteins in their active/native form by elution from the UbiQapture™-Q matrix using high salt buffer.

Ordering information: Quantity: Provides sufficient material for 20 x 20μl reactions. Contents:
UbiQapture™-Q matrix (Prod. No. BML-UW0125) 0.5 ml settled resin provided; 50% suspension.
Control ubiquitinylated-protein lysate (Ub-lysate) (Prod. No. BML-UW0130) 500 µg (100µl), 5mg/ml in 50mM HEPES, pH 7.6, 1mM DTT.
Ubiquitin-conjugates specific HRP-linked antibody solutions (Prod. No. BML-KW0150) 25µl; HRP-linked antibody for use with ECL western blotting detection reagents.
0.5ml screw capped tube pack (Prod. No. BML-UW0135) 25 tubes.
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