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NEDDylation Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences

UW0590-0001

89165-182EA 531.08 USD

89165-182

NEDDylation Kit, Enzo Life Sciences

Protein Linking and Labeling Systems

This kit provides the means of generating a thioester-linked NEDD8-conjugated E2 enzyme, utilizing the first two steps in the NEDD8 cascade, for use in the NEDDylation of E3 ligases and target substrate proteins (user supplied)

This kit provides sufficient material for 20 x 20µL reactions
Generate NEDD8-E2 thioesters for use in the in vitro NEDDylation of target proteins
Demonstrate novel proteins are potential targets for NEDDylation
Study NEDD8 activation (E1) and conjugation (E2) steps in the NEDDylation cascade
Generate substrates for deNEDDylating enzymes
Test proteins for NEDD8 E3 ligase activity
Avoid freeze/thaw cycles

Note: This Kit does not contain any NEDD8 E3 ligases.

A NEDD8-specific antibody is provided for detection of NEDDylated proteins via SDS-PAGE and Western blotting.

Delivery information: NEDD8 Activating Enzyme Solution (E1), UbcH12 Solution (E2), NEDD8 Solution NEDDylation Buffer Mg-ATP Solution, NEDD8 Antibody Solution, Non-reducing Gel Loading Buffer.

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