Ubiquitin binding entities, sampler pack, Enzo Life Sciences

UW0120-0001
89165-104EA 698 USD
89165-104
Ubiquitin binding entities, sampler pack, Enzo Life Sciences
Isolation and detection of the full range of ubiquitinylated protein conjugates from a specific cell/tissue lysate. Capture and analysis of specific ubiquitinylated protein conjugates of interest from particular cell/tissue lysates. Pull down of ubiquitinylated proteins from in vitro assays facilitating their semi-quantitative analysis. Separation of ubiquitinylated/non-ubiquitinylated forms of specific proteins of interest. Release of free proteins in their active/native form by cleavage of ubiquitin/ubiquitin chains from the matrix using a deubiquitinylating enzyme. Release of ubiquitinylated proteins in their active/native form by elution from the matrix using high salt buffer.

The UBE Sampler Kit provides a range of UBE agarose conjugates for use in such experiments and for investigation of UBE:Ubiquitin interactions.
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