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FLUOR DE LYS®-SIRT2 Deacetylase Substrate, Enzo Life Sciences

Supplier: Enzo Life Sciences
Fluor de Lys®-SIRT2 is a fluorogenic peptide substrate for SIRT2 (human Sirtuin 2). Based on residues 317-320 of p53 (Gln-Pro-Lys-Lys(Ac)), a site of regulatory acetylation by the PCAF acetyltransferase (lysine 320), it was the best for SIRT2 from among a panel of substrates patterned on p53, Histone H3, and Histone H4 acetylation sites. Fluor de Lys®-SIRT2 is deacetylated by SIRT2 at a rate of more than 50-fold that of the acetylated lysine substrate, Fluor de Lys® (BML-KI104; acetylated substrates both at 25 µM, 500 µM NAD+). Must be used in conjunction with Fluor de Lys® Developer II (BML-KI176). Sufficient for 100 assays of human recombinant SIRT2 (BML-SE251; 2U/well, 100 µM substrate).



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Size Supplier No. VWR Catalog Number Unit Price Quantity
0.5 µmoL BML-KI179-0005 89160-350 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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Purity ≥95% (HPLC)
Formulation Supplied as a 5mM solution (100 µl) in HDAC Assay Buffer.
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