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Sp1 protein (human), (recombinant) (HA-tag), Enzo Life Sciences

Supplier: Enzo Life Sciences
Produced in insect cells (Schneider SL2 cells). Human Sp1 is fused to a HA- and a FLAG®-tag (H. Braun and G. Suske; Biotechniques 26, 1038 (1999)).

The ubiquitously expressed Sp1 was the first identified and cloned member of the Sp-family of mammalian transcription factors. The 785 aa human protein contains an 81 aa DNA-binding domain consisting of three C2H2-type zinc fingers close to the C-terminus. Two glutamine-rich activation domains are present adjacent to serine/threonine stretches in the N-terminal part of the protein. Sp1 recognizes specifically G-rich elements such as the GC-box (GGGGCGGGG) and the GT/CACC-box (GGTGTGGGG) found in housekeeping as well as in many tissue-specific and viral genes.The protein may be employed in the following fields of research:i) Promoter studies: Identification of Sp1 binding promoter elements by bandshift or DNase1 footprint assays.ii) Detection of Sp1 interacting proteins.

Ordering information: Quantity: Sufficient for performing at least 25 protein-DNA interaction experiments in 10mM HEPES, pH 7.9, 0.1mM EDTA, 8.5% glycerol, 170mM KCl, 1µM ZnSO4, 37.5µg/ml Poly dIdC, 10mM DTT and 1mg/ml BSA, analyzed by gel shift assay.



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Size Supplier No. VWR Catalog Number Unit Price Quantity
50 µL 201-106-R050 89144-106 Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

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Protein/Peptide Name Sp1 protein (HA-tag)
Protein/Peptide Type Recombinant
Species Human
Formulation Liquid. In 50mM TRIS-HCl, pH 7.9, containing 420mM KCl, 0.1% NP-40, 12.5mM MgCl2 and 20% glycerol.
Protein Type Recombinant
Specific Activity 0.5BFU/µl (band forming units). 1BFU is sufficient to generate a strong band shift with a labelled oligonucleotide.
Purification Nuclear extract.
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