Keep my session open?
Ending In 
Your shopping session has expired.
Your session has expired. For your security, we have logged you out.
Would you like to log in again?

Sp1 protein (human), (recombinant) (HA-tag), Enzo Life Sciences

Supplier: Enzo Life Sciences
Produced in insect cells (Schneider SL2 cells). Human Sp1 is fused to a HA- and a FLAG®-tag (H. Braun and G. Suske; Biotechniques 26, 1038 (1999)).

The ubiquitously expressed Sp1 was the first identified and cloned member of the Sp-family of mammalian transcription factors. The 785 aa human protein contains an 81 aa DNA-binding domain consisting of three C2H2-type zinc fingers close to the C-terminus. Two glutamine-rich activation domains are present adjacent to serine/threonine stretches in the N-terminal part of the protein. Sp1 recognizes specifically G-rich elements such as the GC-box (GGGGCGGGG) and the GT/CACC-box (GGTGTGGGG) found in housekeeping as well as in many tissue-specific and viral genes.The protein may be employed in the following fields of research:i) Promoter studies: Identification of Sp1 binding promoter elements by bandshift or DNase1 footprint assays.ii) Detection of Sp1 interacting proteins.

Ordering information: Quantity: Sufficient for performing at least 25 protein-DNA interaction experiments in 10mM HEPES, pH 7.9, 0.1mM EDTA, 8.5% glycerol, 170mM KCl, 1µM ZnSO4, 37.5µg/ml Poly dIdC, 10mM DTT and 1mg/ml BSA, analyzed by gel shift assay.



Log in to see your contract pricing and availability.
  Remember me on this device
 

ORDER

Expand All / Collapse All
Size Supplier No. VWR Catalog Number Unit Price Quantity
50 µL 201-106-R050 89144-106 Each Retrieving Restricted
restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
  • State issued document with your organization's Resale Tax ID Number
  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

Generate Barcodes Barcode Label Format: 
Generate PDF Catalog Page

SPECIFICATIONS

Protein/Peptide Name Sp1 protein (HA-tag)
Protein/Peptide Type Recombinant
Species Human
Formulation Liquid. In 50mM TRIS-HCl, pH 7.9, containing 420mM KCl, 0.1% NP-40, 12.5mM MgCl2 and 20% glycerol.
Protein Type Recombinant
Specific Activity 0.5BFU/µl (band forming units). 1BFU is sufficient to generate a strong band shift with a labelled oligonucleotide.
Purification Nuclear extract.
Enter Details

Select the article for which you are looking for Certificate

Indicate the Lot Number. This field is mandatory.

Enter Certificate Details

Select Product:

Enter the Lot number:

Choose from recent batch/lot numbers:

Or
Review & Compare Alternatives
We found alternative products that can save you up to  per item-unit.  To compare product details, select up to 3 alternatives below and click Compare Selected. To add items to your basket, enter a quantity and click Add to Basket.

Original Product:

Description Catalog Number Availability Unit Your Price Price Per Qty