Direct cAMP ELISA Kit, Enzo Life SciencesSupplier: Enzo Life Sciences
101105-988EA 495.53 USD101105-988
Direct cAMP ELISA Kit, Enzo Life Sciences
This direct cAMP ELISA kit is the most sensitive colorimetric kit for intracellular cAMP quantification
- Highly sensitive with an optional acetylation protocol
- High throughput format
- Flexible assay
- Easy to use pre-coated plate and liquid color coded reagents reduce errors
This is a colorimetric competitive immunoassay kit for the quantitative determination of intracellular cAMP in cells or tissues lysed in 0.1 M HCl, which is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased 10-fold by acetylation (reagents included). Absorbance is read at 405 nm. The large signal-to-background ratio offers superior sensitivity compared to competitors. This kit easily assess adenylyl cyclase activation by GPCRs.
For the quantitative determination of cAMP in cell lysates and tissue from any species. Cited sample types include cell culture supernatant and tissue culture.
Delivery information: Kit contains GxR IgG microtiter plate, conjugate, antibody, 0.1M HCl, neutralizing reagent, wash buffer concentrate, standard, pNpp Substrate, stop solution, acetic anhydride, triethylamine.