PerfeCTa® qPCR FastMix® II, Low ROX™, QuantaBio

Supplier: Quantabio

95120-012 95120-05K 95120-250 95120-012 95120-05K 95120-250
97066-002EA 806.36 USD InStock
97066-002 97066-004 97066-006 CA97066-002 CA97066-004 CA97066-006
PerfeCTa® qPCR FastMix® II, Low ROX™, QuantaBio
Nucleic Acid Reagents PCR Reagents Mastermixes
PerfeCTa FastMix II, Low ROX is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments

A key component of PerfeCTa FastMix II, Low ROX is an ultra pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. PerfeCTa FastMix II, Low ROX is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in PerfeCTa FastMix II, Low ROX is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification. Real-Time PCR Instrument Compatibility:
Applied Biosystems 7500
Applied Biosystems 7500 Fast
Stratagene Mx3000P®
Stratagene Mx3005P™
Stratagene Mx4000™
Applied Biosystems ViiA 7
Applied Biosystems QuantStudio™ 12K Flex

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, AccuVue blue qPCR dye, and stabilizers

Storage conditions:
PerfeCTa FastMix II, Low ROX is stable for 2 years when stored in a constant temperature freezer at –20°C. For convenience, it may be stored unfrozen at 2 to 8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing does not affect PCR performance.

Precautions and Disclaimer:
For research use only. Not intended for any animal or human therapeutic or diagnostic use. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa FastMix II is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.
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