PerfeCTa FastMix II is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments
A key component of PerfeCTa FastMix II is an ultra pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA. PerfeCTa FastMix II is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in PerfeCTa FastMix II is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification. Real-Time PCR Instrument Compatibility:
Roche LightCycler™ 480
Qiagen/Corbett Rotor-Gene® Q
Qiagen/Corbett Rotor-Gene® 6000
Qiagen/Corbett Rotor-Gene® 3000
Eppendorf Mastercycler® ep realplex
Eppendorf Mastercycler® ep realplex2 s
Bio-Rad/MJ Opticon 2
Illumina Eco qPCR
Thermo Scientific PikoReal Cycler
BioRad iCycler iQ™
2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, AccuVue blue qPCR dye, and stabilizers
PerfeCTa FastMix II is stable for 2 years when stored in a constant temperature freezer at –20°C. For convenience, it may be stored unfrozen at 2 to 8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing does not affect PCR performance.
Precautions and Disclaimer:
For research use only. Not intended for any animal or human therapeutic or diagnostic use. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa FastMix II is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.