PerfeCTa® qPCR ToughMix®, Low ROX™, QuantaBio

Supplier: Quantabio
95114-05K 95114-250 95114-05K 95114-250 95114-012 95114-012
97065-968EA 3661.08 USD
CA97065-968 CA97065-970 97065-968 97065-970 CA97065-966 97065-966
PerfeCTa® qPCR ToughMix®, Low ROX™, QuantaBio
Nucleic Acid Reagents qPCR/RT-qPCR Enzymes and Kits
PerfeCTa qPCR ToughMix, Low ROX is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues

A key component of PerfeCTa qPCR ToughMix, Low ROX is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. PerfeCTa qPCR ToughMix, Low ROX delivers exceptional performance with either fast or conventional PCR cycling protocols. Real-Time PCR Instrument Compatibility:
Applied Biosystems 7500
Applied Biosystems 7500 Fast
Stratagene Mx3000P®
Stratagene Mx3005P™
Stratagene Mx4000™
Applied Biosystems ViiA 7
Applied Biosystems QuantStudio™ 12K Flex

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, AccuVue blue qPCR dye, and stabilizers

Storage conditions:
PerfeCTa qPCR ToughMix, Low ROX is stable for 2 years when stored in a constant temperature freezer at –20°C. For convenience, it may be stored unfrozen at 2 to 8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing does not affect PCR performance.

Precautions and Disclaimer:
For research use only. Not intended for any animal or human therapeutic or diagnostic use. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

It is a versatile and robust real-time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa qPCR ToughMix, Low ROX contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.
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