PerfeCTa® qPCR ToughMix®, QuantaBioSupplier: Quantabio
95112-250 95112-05K 95112-012 95112-05K 95112-012 95112-250
97065-958EA 307.81 USD InStock97065-958 97065-956 CA97065-954 CA97065-956 97065-954 CA97065-958
PerfeCTa® qPCR ToughMix®, QuantaBio
Nucleic Acid Reagents PCR Reagents Mastermixes
PerfeCTa qPCR ToughMix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues
A key component of PerfeCTa qPCR ToughMix is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. PerfeCTa qPCR ToughMix delivers exceptional performance with either fast or conventional PCR cycling protocols.
PerfeCTa qPCR ToughMix is compatible with many Real-Time PCR Instruments, such as the Roche LightCycler™ 480, Qiagen/Corbett Rotor-Gene® Q, Qiagen/Corbett Rotor-Gene® 6000, Qiagen/Corbett Rotor-Gene® 3000, Eppendorf Mastercycler® ep realplex, Eppendorf Mastercycler® ep realplex2 s, Cepheid SmartCycler®, Bio-Rad CFX96™, Bio-Rad CFX384™, Bio-Rad/MJ Opticon®, Bio-Rad/MJ Opticon 2, Bio-Rad/MJ Chromo4™, Bio-Rad MiniOpticon™, Illumina Eco qPCR, Thermo Scientific PikoReal Cycler, BioRad iCycler iQ™, BioRad MyiQ™ and BioRad iQ™5.
PerfeCTa qPCR ToughMix is stable for 2 years when stored in a constant temperature freezer at –20°C. For convenience, it may be stored unfrozen at 2 to 8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing does not affect PCR performance.
It is a versatile and robust real-time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa qPCR ToughMix contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.
Ordering information: PerfeCTa qPCR ToughMix contains 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, AccuVue blue qPCR dye and stabilizers. For research use only. Not intended for any animal or human therapeutic or diagnostic use. Please consuly the Material Safety Data Sheet for information regarding hazards and safe handling practices.