PerfeCTa® MultiPlex qPCR SuperMix, Low ROX™

Supplier: Quantabio
95108-01K 95108-200 95108-200 95108-050 95108-050 95108-01K
97065-232EA 2366.24 USD
97065-232 CA97065-230 97065-230 97065-228 CA97065-228 CA97065-232
PerfeCTa® MultiPlex qPCR SuperMix, Low ROX™
Nucleic Acid Reagents qPCR/RT-qPCR Enzymes and Kits
PerfeCTa® Multiplex qPCR SuperMix, Low ROX is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers, probe(s), and template for real-time quantitative PCR on Applied Biosystems 7500 or Stratagene MX series of real-time PCR systems

Real-Time PCR Instrument Compatibility:
Applied Biosystems 7500
Applied Biosystems 7500 Fast
Stratagene Mx3000P®
Stratagene Mx3005P™
Stratagene Mx4000™
Applied Biosystems ViiA 7
Applied Biosystems QuantStudio™ 12K Flex

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP) AccuStart Taq DNA Polymerase, ROX Reference Dye (for 580-585 nm excitation), and stabilizers.

Storage conditions:
PerfeCTa® Multiplex, Low ROX qPCR SuperMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. Repeated freezing and thawing of the supermix is not recommended.

Precautions and Disclaimer:
For research use only. Not intended for any animal or human therapeutic or diagnostic use. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa® Multiplex qPCR SuperMix, Low ROX delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations. A key component of this supermix is AccuStart™ Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
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