6 X fidelity of standard taq dna polymerase.
AccuStart Taq DNA Polymerase HiFi is an enzyme mixture of recombinant Taq DNA polymerase preparation, a thermal stable DNA polymerase with 3’→5’ exonuclease activity, and monoclonal antibodies that bind to the polymerase and keep it inactive before PCR thermal cycling (1). Upon heat activation (1 minute at 94ºC), the antibodies denature irreversibly, releasing fully active DNA polymerase. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. This enzyme mixture and optimized HiFi PCR buffer improves the fidelity of DNA synthesis approximately 6-fold higher than Taq DNA polymerase alone and enables amplification of DNA fragments up to 20-kb long (2). AccuStart Taq DNA Polymerase HiFi is a robust alternative PCR enzyme for both routine PCR applications as well as amplification of problematic templates.
Contents:
- AccuStart Taq DNA polymerase HiFi 5 units/µL in 50% glycerol, 20 mM Tris-HCl, 40 mM NaCl, 0.1 mM EDTA, and stabilizers.
- HiFi PCR Buffer (10X) 600 mM Tris-SO4 (pH 8.9), 180 mM (NH4)2SO4
- 50 mM magnesium sulfate 50 mM MgSO4
Storage conditions:
AccuStart Taq DNA polymerase HiFi is stable for 2 years when stored in a constant temperature freezer at 20ºC
Precautions and Disclaimer:
For research use only. Not intended for any animal or human therapeutic or diagnostic use. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
