Buffer Solution, TSS, Alfa Aesar

Supplier: Alfa Aesar
J62318-EQE J62318-EQE
AAJ62318-EQEEA 57.04 USD InStock
CAAAJ62318-EQE AAJ62318-EQE
Buffer Solution, TSS, Alfa Aesar
Buffers pH Buffers
TSS (transformation and storage solution) buffers can be used to make chemically competent cells.

  • Contains 85% LB medium, 10% (w/v) PEG MW 8000, 5% (v/v) DMSO, 50 mM magnesium chloride
  • Fully miscible in water
  • Sterile filtered

TSS solution is used in the one-step procedure for the preparation of competent E. coli and allows transformation of cells without heat-shock. Transformation efficiencies of 106 to 108/µg DNA are typically obtained using TSS.
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