Proteinase K

Supplier: Thermo Scientific
J76230-MC
101175-854EA 120.77 USD
101175-854
Proteinase K
Enzymes
Used in the isolation of native, high molecular weight DNA and RNA.

Source: Tritirachium album.

Proteinase K is a highly active and stable endopeptidase used in a wide range of applications purified from the fungus T. album. It cleaves peptide bonds mainly following the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids(1) and is classified as a serine protease. Proteinase K is useful in purifying high molecular weight nucleic acids from cells and tissues (i.e. genomic DNA isolation from mouse tails) and in revealing protein structure and function.

References:
1. EBELING, W., HENNRICH, N., KLOCKOW, M., METZ, H., ORTH, H. D. AND LANG, H. (1974) Eur. J. Biochem. 47, 91-97.
5. ROELCKE, D. AND ULENBRUCK, G. (1969) Z. Med. Mikrobiot. und Immunol. 155, 156-170.
6. BRDICZKA, D. AND KREBS, W.(1973) Biochem. Biophys. Acta 297, 203-212.
7. WILLIAMSON, J., GREENE, J., CHERIF, S. AND MILNER-WHITE, E. J. (1977) Biochem. J. 167, 731-737.

Used as a general protease to inactivate DNases, RNases and to degrade proteins. Specifically modifies cell surface proteins and glycoproteins for analysis of membrane structures for protein localization(5,6). Produces characteristic protein fragments used in enzyme/protein structure and function studies(7).
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