Phenol : chloroform : iso-amyl alcohol (25:24:1), ultrapure Molecular biology gradeSupplier: Thermo Fisher Scientific Chemicals Inc.
Used for nucleic acid extraction and purification.
A liquid mixture of phenol, ACS Reagent Grade chloroform and ACS Reagent Grade isoamyl alcohol in a ratio of 25:24:1 supplied with an additional Alkaline Equilibration Buffer used to adjust the pH to 8.0.
Note: Phenol:Chloroform:Isoamyl Alcohol (25:24:1) is packaged at pH 6.7, the product is accompanied by a separate Alkaline Equilibration Buffer. When ready to use add the supplemental buffer's entire contents to the Phenol:Chloroform:Isoamyl Alcohol, then mix or shake, and allow to separate. Once this is completed the product will be at a pH of 8.0.
Phenol/Chloroform RNA Isolation Method
A. Guanidine HCl Homogenization Buffer for RNA Isolation: 8.0M Guanidine HCl, 0.1M Sodium Acetate, pH 5.5, 5.0mM Dithiothreitol, 0.5% Sodium Lauryl Sarcosinate
B. Extraction with Phenol: Most extractions are performed using equal volumes of equilibrated phenol with respect to the sample volume. Normally, the extraction is performed twice with phenol and once with a 1:1 solution of phenol/chloroform. Hence, it is estimated that 25ml of phenol is required for every 10ml of sample.
Note: If regular phenol is purchased, it will need to be equilibrated before use. For this reason, many customers may prefer to purchase phenol from USB that is already equilibrated (Product Code 101174-858 or 101172-024).
C. RNA Resuspension Buffer: TE, pH 7.5.
CAS Number: 136112-00-0