Mouse monoclonal [28F2] to Nop1p - Nucleolar Marker
IF: Use at a dilution of 1/2000 - 1/20000 (yeast cells). IP: Use 2-4 ?l for an amount of lysate derived from 5 OD600 units of yeast culture. WB: Use at a dilution of 1/10000 (cell lysates)- 1/25000 (nuclear fractions)(yeast protein samples). Predicted molecular weight: 34 kDa. For other (non-ECL) western detection methods, use at a dilution of 1/1000 - 1/5000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: NOP1
Clonality: Monoclonal
Clone: 28F2
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG3
Reactivity: Yeast