The affinity purified anti IgG H and L reactes with all subclasses of IgG and with all classes of mouse immunoglobulins by immunodiffusion and IEP techniques due to the presence of anti light chains. In a direct ELISA against purified mouse immunoglobulins, conjugate diluted to 1/50,000 produced an O.D.=1.0 in a 15 minute reaction with TMB as substrate. Optimal conjugate working dilutions should be determined for your particular assay conditions.. Goats were immunized with purified mouse IgG isolated from pooled normal mouse serum. The anti IgG H and L chains were immunoaffinity purified off an IgG-containing solid phase immunoabsorbant and conjugated with horseradish peroxidase (HRP)
Type: Secondary
Antigen: IgG
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope: Heavy and Light chain
Host: Goat
Isotype: IgG
Reactivity: Mouse