There are two kinds of IGF receptors based on their relative affinities for IGFI versus IGFII. The IGFI receptor prefers IGFI over IGFII and weakly binds insulin. IGFI receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kD) and two beta (95 kD) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGFI receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of the IGFI receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGFI receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGFI and II have been shown to be mediated via the IGFI receptor.
For ELISA: Use at a concentration of 2 - 2.5?g/ml. For Neut: Use at a concentration of 10 - 12?g/ml. Neutralizes ~50-75% of IGF-I receptor-mediated rhIGF-I biological activity in MCF7 cells. Will block cell surface IGF-I receptor mediated IGF-I and IGF-II bioactivities. For WB: Use at a concentration of 1 - 2 ?g/ml under non reducing conditions. Predicted molecular weight: 155 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Type: Primary
Antigen: IGF1R
Clonality: Monoclonal
Clone: 33255.111
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human
