Fas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by Fas Ligand or an anti-Fas cross-linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumours. Tumour cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system. Fas is believed to be involved in various autoimmune diseases including ulcerative colitis, Graves disease, and rheumatoid arthritis. Fas expression on gastric epithelial cells in patients infected with H. Pylori is responsible for the accelerated apoptosis of the cells. Serum Fas Ligand concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension.
Peptide ELISA: antibody detection limit dilution 1:2000.FACS: Use at an assay dependent dilution. ICC/IF: Use at a concentration of 10 µg/ml.
Western blot: Approx 35kDa band observed in lysates of Human Lung, in Mouse and Rat Thymus, and in cell line K562 (calculated MW of 37.3kDa according to NP_068779.1). Recommended concentration: 1-3µg/ml.Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: Fas
Clonality: Monoclonal
Clone: H11
Conjugation: Biotin
Epitope:
Host: Rat
Isotype: IgG2a
Reactivity: Human, Mouse, Rat
