E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F 3 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides.
GSA: Use at an assay dependent dilution. IP (denatured): Use at an assay dependent dilution. WB: Use at a concentration of 4 - 8 ?g/ml. This concentration is determined using whole extract of transfected 293T (human embryonal kidney) cells expressing E2F3. Detects a band of approximately 57 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: E2F3
Clonality: Monoclonal
Clone: PG30
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a
Reactivity: Human