Goat polyclonal to TNF beta
ELISA: Use at a concentration of 1 ?g/ml to detect 0.6 ng/well of rhTNF beta. Neut: Use at a concentration of 0.1 - 1.6 ?g/ml. WB: Use at a concentration of 2 ?g/ml to detect rhTNF beta at 5 ng/lane. Predicted molecular weight: 22 kDa. Anti-Human TNF beta is tested for its ability to neutralize the biological activity of rhTNF beta on L929 cells. The ND50 of the antibody is defined as the concentration of antibody required for one-half maximal inhibition of bioactivity of rhTNF beta which is present at 5 times its own EC50 (the concentration of rhTNF beta producing a one-half maximal bioactivity without antibody). In this bioassay, rhTNF beta was pre-incubated with various dilutions of the antibody in culture medium containing actinomycin D for 1 hour at 37°C in a 96-well microtiter plate. Following this pre-incubation period, the antibody-antigen mixture was added to confluent cultures of L929 cells in 96-well microtiter plates to give a final concentration of 1 ng/ml rhTNF-beta and 1 ug/ml actinomycin D in 0.1 ml. This was incubated for 24 hours at 37°C in a 5% CO2 humidified incubator and then fixed with 5% formaldehyde and stained with crystal violet. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: TNFB
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Goat
Isotype: IgG
Reactivity: Human
