You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity

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Catalog Number: (PAC8820)
Supplier: Promega Corporation
Description: The Flexi Vector System provides a rapid, efficient, high-fidelity way to transfer protein-coding regions without the need to resequence.

Catalog Number: (PAC1263)
Supplier: Promega Corporation
Description: For ligation of DNA ends.

Supplier: New England Biolabs (NEB)
Description: A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hyd...

Catalog Number: (102877-808)
Supplier: New England Biolabs (NEB)
Description: The NEBuilder HiFi DNA Assembly Cloning Kit combines the power of the NEBuilder HiFi DNA Assembly Master Mix with NEB 5-alpha Competent E

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Supplier: New England Biolabs (NEB)
Description: Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer.

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Catalog Number: (PAM1794)
Supplier: Promega Corporation
Description: For ligation of DNA ends, mutagenesis and cDNA cloning

Catalog Number: (102715-912)
Supplier: New England Biolabs (NEB)
Description: The Gibson Assembly Cloning Kit combines the power of the Gibson Assembly Master Mix with NEB 5-alpha Competent E.coli, enabling successful assembly o...

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Catalog Number: (102877-596)
Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 1, High Concentration (M0437M), is a 3X higher concentration than our standard T4 RNA Ligase 1 (M0204S/L)

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Catalog Number: (103218-906)
Supplier: New England Biolabs (NEB)
Description: NEBuilder HiFi DNA Assembly was developed to improve the efficiency and accuracy of DNA assembly

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Catalog Number: (PAP9801)
Supplier: Promega Corporation
Description: Used for high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, if the sequence contains a rib...

Catalog Number: (102855-130)
Supplier: New England Biolabs (NEB)
Description: The NEBNext® Ultra™ Ligation Module is designed for use with the NEBNext Singleplex or Multiplex Oligos for Illumina.

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Catalog Number: (200064-420)
Supplier: New England Biolabs (NEB)
Description: 9°N™ DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleoti...

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Catalog Number: (103307-256)
Supplier: New England Biolabs (NEB)
Description: RtcB Ligase is expressed as a His-tagged fusion in E. coli.

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Catalog Number: (103753-176)
Supplier: INTACT GENOMICS, INC MS
Description: Taq ligase is thermostable DNA ligase from the thermophilic bacterium Thermus aquaticus cloned to E. coli cells.


Supplier: INTACT GENOMICS, INC MS
Description: Intact Genomics propriety Ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR pro...

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