You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Supplier: New England Biolabs (NEB)
Description: The Quick Blunting Kit is used to convert DNA with incompatible 5´ or 3´ overhangs to 5´ phosphorylated, blunt-ended DNA for efficient blunt-end ligat...

Small Business Enterprise

Supplier: TELESIS BIO
Description: Generate seamless plasmid DNA or BAC clones without restriction digestion

SDS

Catalog Number: (102877-564)
Supplier: New England Biolabs (NEB)
Description: This PCR Cloning Kit contains an optimized 2X Cloning Master Mix with a proprietary ligation enhancer and a linearized vector that uses a novel mechan...

SDS Small Business Enterprise


Supplier: Agilent Technologies
Description: The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve...
Supplier: TELESIS BIO
Description: Generate seamless plasmid DNA or BAC clones without restriction digestion

SDS

Supplier: TELESIS BIO
Description: Generate seamless plasmid DNA or BAC clones without restriction digestion

SDS

Supplier: Agilent Technologies
Description: The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cl...

SDS

Supplier: Agilent Technologies
Description: The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activi...

Supplier: Agilent Technologies
Description: For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency a...

Catalog Number: (99902-306)
Supplier: Agilent Technologies
Description: Pfu DNA ligase is a recombinant enzyme derived from Pyrococcus furiosus hyperthermophilic marine archaebacterium that catalyzes linkage ...


Supplier: TELESIS BIO
Description: Generate seamless plasmid DNA or BAC clones without restriction digestion

SDS

Supplier: New England Biolabs (NEB)
Description: This NEB Golden Gate Assembly Kit (BsaI-HFv2) contains an optimized mix of BsaI-HFv2 and T4 DNA Ligase. Together these enzymes can direct the assembly...

Catalog Number: (101417-934)
Supplier: New England Biolabs (NEB)
Description: NEB offers the Quick Blunting™ and Quick Ligation™ Kits together as a bundle at a value price.

Small Business Enterprise


Supplier: Rockland Immunochemical
Description: DNA Ligase T4 Molecular Biology Grade

Catalog Number: (102969-096)
Supplier: New England Biolabs (NEB)
Description: This PCR Cloning Kit contains an optimized Cloning Mix containing a proprietary ligation enhancer and a linearized vector that uses a novel mechanism ...

Small Business Enterprise


Catalog Number: (95040-312)
Supplier: Cytiva
Description: T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fr...


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