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PerfeCTa® SYBR® Green SuperMix Reaction Mixes, QuantaBio

Supplier: Quantabio
Unique buffer and stabilizers have been optimized exclusively for SYBR® Green I qPCR to deliver maximum PCR efficiency, sensitivity, and a robust fluorescent signal. Highly specific amplification is crucial to successful qPCR with SYBR® Green I technology because this dye binds to and detects any dsDNA generated during amplification. A key component of this supermix is AccuStart™ Taq DNA polymerase with monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Kits are available for all real-time PCR instrument platforms including those requiring normalization with ROX™ reference dye. Platforms not requiring passive reference dye include Roche LightCycler® 400, Bio-Rad® iQ™, MyiQ™, iQ™5, Opticon®, and Chromo4™, Corbett Rotor-Gene®, Eppendorf® Mastercycler®, and Cepheid® SmartCycler®. Kits with Low ROX™ reference dye are for use with platforms including AB 7500 and Stratagene® Mx™. Kits with ROX™ reference dye are for use with platforms including AB 7000, 7300, 7700, 7900, and StepOne™.

All SuperMixes are available with or without uracil-N-glycosylase (UNG) for prevention of carry-over contamination from previous dU containing PCRs.

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Reactions per Kit Supplier No. VWR Catalog Number Unit Price Quantity
100 95054-100 101414-150 Each Retrieving Restricted
500 95054-500 101414-152 Each Retrieving Restricted
2000 95054-02K 101414-146 Each Retrieving Restricted

SuperMix for iQ™
Reactions per Kit Supplier No. VWR Catalog Number Unit Price Quantity
100 95053-100 101414-142 Each Retrieving Restricted
500 95053-500 101414-144 Each Retrieving Restricted
2000 95053-02K 101414-138 Each Retrieving Restricted

SuperMix with Low ROX™
Reactions per Kit Supplier No. VWR Catalog Number Unit Price Quantity
100 95056-100 101414-166 Each Retrieving Restricted
500 95056-500 101414-168 Each Retrieving Restricted
2000 95056-02K 101414-162 Each Retrieving Restricted

SuperMix with ROX™
Reactions per Kit Supplier No. VWR Catalog Number Unit Price Quantity
100 95055-100 101414-158 Each Retrieving Restricted
500 95055-500 101414-160 Each Retrieving Restricted
2000 95055-02K 101414-154 Each Retrieving Restricted
restricted In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
  • State issued document with your organization's Resale Tax ID Number
  • City or County issued Business License
  • State Department of Health Services License
  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

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