How to Splice Microbore Tubing

Some specialized autoanalysis procedures require tubing IDs less than 0.51 mm. In these cases, order the Tygon® tubing with Luer lock fittings (95611-series) or make connections via the splicing method.

  1. Bring together one end of a length of color-coded tubing (A) and one end of a length of extension tubing (B). (Both lengths of tubing should have the same ID and OD).
  2. Center a 1" to 2" piece of larger tubing (C) over the ends of the two pieces to be joined (A and B). The short tubing piece should be of similar material and with an ID approximately equal to the OD of the tubing to be joined.
  3. With the short piece (C) centered over the joint, apply solvent or sealant to the outside of tubing (B) and slide tubing (C) onto (B). Repeat the process for tubing (A) to join tubing permanently. If future tubing changes are necessary, then apply solvent or sealant only to tubing (B) and (C). Use a ketone-type solvent to splice Tygon® or Viton® tubing; an RTV adhesive for silicone tubing; and a tetrahydrofuran with PharMed® BPT tubing.