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PEIpro® is a ready to use 1 mg/ml optimised linear polyethylenimine (PEI) reagent for the production of virus & recombinant proteins by Transient Gene Expression (TGE), either in adherent cells in presence of serum or in suspension HEK-293 and CHO cells grown in low serum or serum-free media.
Optimized for Large Scale Transient Gene Expression for virus production
Increased viral titers compared to other PEIs
Perfectly suitable for the development of bioproduction processes
Free of animal-origin components
Well characterized and lot release tested linear PEI
Reliable and secure supply
Can also be used for large scale protein production
PEIpro® undergoes full chemical testing and is compliant with the regulatory guidelines for raw materials used in bioproduction. The reagent is supplied with advanced quality controls including a specific transfection efficiency test that ensures excellent lot-to-lot consistency, and an implied license statement.
With its unique property to tightly condense several different plasmids, PEIpro® is ideal for the co-transfection of multiple plasmids that is often required for virus production. Transfection with PEIpro® ensures high viral titers in most commonly used virus producing and packaging cell lines (HEK-293, VERO, CAP, CAP T, etc.). PEIpro® is well suited for production of all types of viruses, achieving for example viral titers > 107 IG/ml for lentiviruses and > 1011 to 1012 VG/ml for AAV.
Compared to CaPO4, PEIpro® is more robust with transfection efficiencies ranging from 70 to 90% both in adherent and in suspension cells. It is also more powerful as it gives higher viral titers using lower DNA amount.
With PEIpro®, transfection processes are easy to scale up. PEIpro® can be used from R&D to large scale virus or protein production. With a given 2 year shelf life and a ready to use formulation, PEIpro® is the reagent of choice for virus production runs in most cell culture media and format (Table 1) using both adherent and suspension cells.
Examples of citations
Melidoni, A. N., Dyson, M. R., McCafferty, J. (2016). Selection of Antibodies Interfering with Cell Surface Receptor Signaling Using Embryonic Stem Cell Differentiation. Methods Mol Biol 1341, 111-32.
Stockinger, L. W., Eide, K. B., Dybvik, A. I., Sletta, H., Varum, K. M., Eijsink, V. G., Tondervik, A., Sorlie, M. (2015). The effect of the carbohydrate binding module on substrate degradation by the human chitotriosidase. Biochim Biophys Acta 1854, 1494-501.
Bodnar, E., Raymond, C., Lopez, P. G., Villacres, C., Butler, M., Schoenhofen, I. C., Durocher, Y., Perreault, H. (2015). Mass spectrometric analysis of products of metabolic glycan engineering with azido-modification of sialic acids. Anal Bioanal Chem 407, 8945-58.
Li, S. K., Abbas, A. K., Solomon, L. A., Groux, G. M., DeKoter, R. P. (2015). Nfkb1 activation by the E26 transformation-specific transcription factors PU.1 and Spi-B promotes Toll-like receptor-mediated splenic B cell proliferation. Mol Cell Biol 35, 1619-32.